Description
Application: RNA staining of E. coli with T-Fluor 480
- Cultivate E. coli BW25113 on LB medium at 37°C overnight.
- Add 1/100 volume of culture medium to LB medium with or without 100 µg/mL rifampicin (*) and incubate at 37°C for 4 hours.
- After removing the supernatant by centrifugation, add PBS containing 6.5 μg/mL T-Fluor 480 to the pellet and stain at room temperature for 5 minutes.
- Observed under a fluorescence microscope using a filter for GFP.
* Rifampicin: RNA synthesis inhibitor
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